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Gas Chromatography-Mass Spectrometry (GC-MS) is often employed for stable isotope tracing studies due to its ability to separate and quantify metabolite fragments
aeruginosa to the mucoid phenotype worsens prognosis although it is more common in patients with CF.74,75 Although there are no studies to guide practice following the first isolation, an attempt to eradicate seems pragmatic for what it should be treated aggressively regardless of the patient's clinical signs or symptoms in order to eliminate the microorganism from the sputum as it is very difficult to do so once chronic colonization is established.3,4 In non-CF BE the strategy proposed for eradication is oral ciprofloxacin for 3 weeks and if it fails, the same protocol as for CF is recommended (nebulized antibiotic: tobramycin, sodium colistimethate or aztreonam lysine) plus oral ciprofloxacin for 3 weeks, followed by inhaled antibiotics for 312 months.4,60,76 An alternative would be an association of 2 anti-Pseudomonas intravenous antibiotics for 1421 days, followed by an inhaled antibiotic for 312 months.4,60,76 As was stated above, an antibiogram is essential for guiding treatment although it is recognized that in vitro sensitivity does not always correlate with therapeutic response
