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Description

Nofer JR, Levkau B, Wolinska I, Junker R, Fobker M, von Eckardstein A, Seedorf U, Assmann G

Cells were then washed twice in ice-cold mitochondrial isolation buffer (MIB: 20 mM HEPES, 70 mM sucrose, 220 mM mannitol, pH 7.4, 1 mM EDTA, Complete protease inhibitors), resuspended in MIB and lysed using nitrogen cavitation for 5 min at 500 psi