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Gone too soon

Death caused by the absence of Cys was rescued by the addition of free radical scavengers ferrostatin-1 (Fer-1) and N-acetyl-cysteine (NAC) but not by inhibition of caspase-dependent apoptosis by z-VAD-fmk or necroptosis by Nec1s (Fig 3A and B), indicating that Cys starvation induced ferroptosis in ES cells

Researchers utilizing this material should follow institutional procedures for equipment handling, data collection, and controlled analysis, ensuring all work remains within approved research scopes

Since the pad2-1 mutant is severely deficient in GSH content, we supplemented these mutant seedlings with exogenous GSH or DTT

Reverse phase chromatography was performed using an EASY column SC200 (10 cm, inner diameter: 75 m, particle size: 3 m) using a pre-column (EASY column SC001, 2 cm, inner diameter: 100 m, particle size: 5 m) and a mobile phase gradient from 5 to 40% acetonitrile, 0.1% trifluoroacetic acid in water in 150 min (flow rate of 300 nl min 1

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