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Some will cite health reasons, and the National Institute of Health states that a liter of water in a plastic bottle contains an average of 240,000 tiny pieces of plastic, 90% of which are nanoplastics

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It regulates cytokines (TNF-, IL-6), decreases swelling, and limits immune-cell overactivity

Peptides break down quickly in warm environments

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Shaking the vial instead of swirling creates turbulence that can denature peptide chains through mechanical stress