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KleimanNJEdmondsonEFWeilMMFallgrenCMKingASchmidtCet al

Tanzer, R

After the homologous recombination, Amp-r or Kan-r cassette was excised by adding arabinose to the log phase liquid culture of the EL250 cells with the correctly modified BAC at 0.1% v/v for 1 h at 32C to sufficiently induce Flpe (Fig