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Wogonoside seems to alleviate colitis by protecting against intestinal barrier dysfunction through the reinforcement of tight junctions via regulation of the MLCK/pMLC2 signaling pathway in Caco2 cells [105], whereas in DSS-induced UC mice this compound seemed to lead to dual inhibition of NF-B and NLRP3 inflammasome [106]

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Van de Donk et al

GST pull-down takes advantage of GST's affinity for GSH-coupled beads

A 60-s cut-off time was applied to each animal to prevent damage to the paw tissue ( 2.5.3 Open field test The open field test was conducted to evaluate the overall motor activity of the animals