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Abbreviations ALS Amyotrophic Lateral Sclerosis ARE Antioxidant Response Element BCA Bicinchoninic Acid CSF Cerebrospinal Fluid DTT Dithiothreitol ECL Enhanced Chemiluminescence reagent EDTA Ethylenediaminetetraacetic Acid EGTA Ethylene Glycol-bis (2-aminoethylether) N, N,N, N-tetraacetic Acid fALS Familial ALS GPx Glutathione Peroxidase GR Glutathione Reductase GSH Reduced Glutathione GSSG Oxidized Glutathione H 2 DCFDA 2,7-Dichlorodihydrofluorescein Diacetate HPLC High-Performance Liquid Chromatograph HRP Horseradish Peroxidase MDA Malondialdehyde mutSOD1 Mutant SOD1 NAM Nicotinamide Nrf2 Nuclear Factor Erythroid-2-related Factor PBMCs Peripheral Blood Mononuclear Cells PBS Phosphate-Buffered Saline PCA Principal Component Analysis PMSF Phenylmethylsulfonyl Fluoride PVDF Polyvinylidene Difluoride sALS Sporadic ALS SDS Sodium Dodecyl Sulfate SOD1 Superoxide Dismutase 1 Ta Annealing Temperature TBA Thiobarbituric Acid t-BHP tert-Butyl Hydroperoxide undSOD1 Undetermined SOD1 Mutations vit E Vitamin E References [1]. [2]. [3]