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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

commonly reconstituted in sterile water or buffer for research use Mechanism (working): allosteric potentiation of HGF/c-Met signaling

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