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While these approaches show promise in preclinical models, clinical translation faces substantial hurdles: most nanoparticles achieve only modest brain accumulation with heterogeneous parenchymal distribution, and their long-term safety, biodistribution, and immunogenicity remain poorly characterized (192)

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RES also targets NF-B pathways, ameliorating anxiety and depression-like behaviors in stress models and showing greater efficacy than fluoxetine in regulating NF-B/NLRP3 signaling pathways [160,161]

After washing with the 0.1 M phosphate buffer (pH 7) the samples were incubated in intensification solution (0.025 % (v/v) H 2 O 2 and 0.005 % (w/v) CaCl 2 ) and kept at room temperature for 30 min

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