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After 3 h of infection, the cells were washed with 1 PBS and removed from the monolayer using a scraper in 300 L of the lysis buffer (for lipid peroxidation assay) or 500 L of cold 1 PBS (for protein carbonylation assay)

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Moreover, heavy metals can deplete glutathione, a master intracellular antioxidant, by binding to thiol groups or interfering with glutathione synthesis and recycling (Ercal et al., 2001

Real-time RT-PCR in COVID-19 detection: Issues affecting the results