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In previous studies TS was shown to increase cellular and mitochondrial ROS by multiple experimental approaches 9,20

Despite these protective functions, it has been demonstrated that MTs are involved in tumorigenesis, cellular differentiation, drug resistance, and metabolic disorders such as diabetes and obesity, in which MTs expression is substantially deregulated in adipose tissue

The Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), developed by the American Psychiatric Association, defines AUD as a cluster of behavioral and physical symptoms, including withdrawal, tolerance, and craving (American Psychological Association, 2013)

Mitochondrial Localization of PARP1 Requires Interaction With Mitofilin and Is Involved in the Maintenance of Mitochondrial DNA Integrity. Journal of Biological Chemistry 284, no

This is where the protocol really starts to compound

Nature Communications Biology