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After 24 h cultivation in the media/solvent mixtures at the indicated concentrations, the culture medium was changed to another medium supplemented with 33 mg/mL neutral red

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Additive effect on kidney outcomes including macroalbuminuria was also expected from AMPLITUDE-O substudy, however, additive effect of semaglutide and SGLT2i on the composite kidney outcomes without macroalbuminuria was not indicated in the FLOW substudy [50]