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Current markers (e.g., GPX4 expression, 4-HNE, and serum creatinine) lack specificity for ferroptosis-driven cyst progression, as they are altered in other kidney diseases or metabolic disorders

For instance, SeMet significantly increased alanine aminotransferase, aspartate transferase, and lactate dehydrogenase levels in the liver for prolonged periods relative to SeNPs, exhibiting acute liver injury (256)