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Conformational changes due to ligand binding are known to be a major contributor to heat capacity changes 36 , and there is precedence for ligand binding to Bateman modules to diverge from a simple one-site binding model when dimerisation of Bateman modules occurs 37

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(2009) Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles Proteomics, 9, 5425-5436 20

Time Cycles Initial denaturation 98 3 min 1 Denaturation 98 10 sec 15 (decrease by 1 C per cycle) Gradient Annealing 70-55C 20 sec Extension 72 30 sec/kb Denaturation 98C 10 sec 20 Annealing 55C 20 sec Extension 72C 30 sec/kb Final extension 72 5 min 1 4Characteristics of Different PCR Protocols: Protocol Type Speed Specificity PCR Yield Detection Rate Two-Step Fastest High Medium High Three-Step Medium Medium Highest Medium Gradient Annealing Slow High Medium High $15.00 - $3,665.00 2 HotStart Fast PCR Master Mix (With Dye) Product description 2 HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency

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