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To quantify the proportion of M2 macrophages, cells were stained with APC anti-mouse CD206 antibodies (BioLegend, USA) and PE-Cy7 anti-mouse CD86 antibodies (BD, Cat No

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340 Research has shown that aging exacerbates multiple sclerosis pathology through several biological mechanisms, which span from increased neuroinflammation and a dysregulated immune system to impaired remyelination and gut dysbiosis

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For example, skipping breakfast for 6 week resulted in a reduction in daily physical activity energy expenditure of approximately 450 kcal/d (Reference Betts, Richardson and Chowdhury40) , with similar findings reported with other methods of severe energy restriction (Reference James, James and Clayton22)

Rouhier, Couturier & Jacquot 2006