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N Eng J Med 2010;362(25):2389-2398 Lebovic DI, Mueller MD, Taylor RN

Nuclear RNA was extracted using Tri-Reagent (9424, Sigma), as described in the RNA-seq section

For patients receiving parenteral nutrition For patients receiving parenteral nutrition Administer IV insulin added to the parenteral nutrition container for patients receiving continuous parenteral nutrition

Both components consist of defined amino acid sequences associated with receptor-mediated endocrine signaling pathways

Tavares MR, Frazao R, Donato J

Insulin-like growth factor I receptor signaling and nuclear translocation of insulin receptor substrates 1 and 2