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To quantitatively measure the expression levels of the 60 genes extracted through microarray analysis, RT-qPCR was used

The tissues were then rinsed and incubated with a biotinylated secondary antibody (1:500, Cat# GB23303, Servicebio, China) and streptavidin-HRP (Cat#SP-9001, ZSGB-BIO, China)

Acta Physiol Plant 31:393400 Soltani Y, Saffari VR, Moud AAM, Mehrabani M (2012) Effect of foliar application of -tocopherol and pyridoxine on vegetative growth, flowering, and some biochemical constituents of Calendula officinalis L

Elevated TNF- levels might impede steroidogenesis under inflammatory conditions and induce germ-cell apoptosis in experimental autoimmune orchitis (Theas et al., 2008)

NMR chemical shift perturbation analysis The CSPs were calculated according to the following equation: where H and N indicate the chemical shift changes of the amide proton and nitrogen, respectively, using CcpNMR 2.5 49