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346 Another astrocyte-specific mechanism involved in ictogenesis is linked to gap junction forming connexion Cx43 channels, which are down-regulated in epilepsy

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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, Mo.) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen, and Kaleidoscope, BioRad)

The hyper-reactive immune system is accompanied by the presence of oxidative stress (OxS) with increased release of reactive oxygen species (ROS)

Further to this, data from naturally aged, healthy wild-type mice provides supporting evidence by also showing an inverse relationship between serum B12 and IL- 6