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The copper sulfate-induced zebrafish inflammation mode was established according to the method of Pereira et al

The BBB prevents almost 98% of small molecule drugs and nearly all large-molecule therapeutics such as recombinant peptides, proteins, anti-sense agents, and genetic vectors, from reaching the brain [1]

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They overexpressed the donated GR and this was supplementing the endogenous GR

Additionally, copper ions in GHK-Cu can catalyze oxidation of certain peptides (such as those containing cysteine residues), leading to cross-reactivity and degradation

All experiments were performed in accordance with the guidelines established by the National Institute of Health Guide for the Care and Use of Laboratory Animals and approved by the University of Texas Institutional Animal Care and Use Committee