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Morphological changes, occurring during the activation of microglia and astrocytes, or higher proliferation of NG2 glia, microglia and astrocytes are all hallmarks of ischemia (Davalos et al., 2005

These findings indicate that alopecia areata is a multifaceted autoimmune disorder characterized not only by hair loss but also by systemic manifestations, a highly variable clinical course, and an unpredictable prognosis

The samples were loaded on the 96-well S-Trap plate (Protifi), placed on top of a deepwell plate, and centrifuged for 2 min at 1,500 x g at RT