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As for the PV2 nanobody, mutants S26pAcF, S71pAcF, and Q82pAcF showed Tm values of 52.2 C, 52.9 C, and 53.1 C, higher than 47.9 C of wild type

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doi: 10.3389/fmed.2026.1786788 Received 13 January 2026 Revised 17 March 2026 Accepted 23 March 2026 Published 10 April 2026 Volume 13 - 2026 Edited by Aleksandra Kuzan, Wrocaw University of Science and Technology, Poland Reviewed by Iryna Popova, Bukovinian State Medical University, Ukraine Nikita Nazarov, OOO PIXEL MED, Russia Updates Copyright 2026 Wei, Li, Liu, Wu, Li, Xu, Yang, Yin, Zhang and Liu

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