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Intracellular glutathione was detected and quantified using the Intracellular glutathione (GSH) Detection Assay Kit (ab112132

The shear stress-induced augmentation of whole-cell TRPM7 current was not affected by an exocytosis-blocking reagent brefeldin A under the experimental conditions employed in this study, although laminar flow-induced shear stress was reported to cause exocytotic translocation of TRPM7 to the plasma membrane in some cell types (Oancea et al., 2006)

The aerodynamic diameter of a particle of arbitrary physical properties dictates where the particle will land in the airways through three mechanisms: inertial impaction, gravitational sedimentation and diffusion 127