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The reaction mixture contained 1 ml of sample and 2 ml of TBA-TCA-HCl reagent (0.37% thiobarbituric acid, 0.25 N hydrochloric acid and 15% trichloroacetic acid), placed in boiling water bath for 15 min, cooled at room temperature and centrifuged for 10 min

3 Small nerve fibers mediate somatic and autonomic functions, an evolutionary link that may reflect visceral defense mechanisms responding to pain as a signal of danger

Belscak A, Bukovac N, Piljac-Zegarac J (2011) The influence of ascorbic acid and honey addition on the antioxidant properties of fruit tea infusions: Antioxidants in fruit tea infusions

Applying heat and performing self-massage also helps mitigate any discomfort felt during this procedure

Volk M, et al