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The medium was removed, cells were placed on ice and lysed with 2x Laemmli sample buffer (BioRad) supplemented with 4% -mercaptoethanol

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Food Chem (2012) 131(3):86978.10.1016/j.foodchem.2011.09.068 99 LiSHanZMaYSongRPeiTZhengTet alHydroxytyrosol inhibits cholangiocarcinoma tumor growth: an in vivo and in vitro study

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Oxidative stress induces a form of programmed cell death with characteristics of both apoptosis and necrosis in neuronal cells