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The NHS schedule for treating B12 deficiency is a loading dose of 1mg hydroxocobalamin by intramuscular injection every two to three days for two weeks, then a maintenance dose every two to three months for life when the cause is non-dietary

The peptide was acquired as a 99% pure substance that was further dissolved in saline prior to the random assignment of male mice to an experimental treatment

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Subcutaneous injection ensures the compound saturates the entire system, allowing it to find and act upon receptor sites far from the administration point

If long-term storage of a reconstituted solution is required, researchers typically aliquot the solution into single-use vials, snap-freeze at -80 C, and thaw only once before use