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MIBI staining, data acquisition, and analysis Slide preparation, staining, antibody optimization, and imaging were performed by the MIBI Core at UCSF

Animal studies utilizing acute stress models have shown that in mice stress-induced BBB permeability reduces the pyridostigmine dose required to inhibit brain acetylcholinesterase by 50% to less than 1/100th of the usual dose used (Friedman et al, 1996)