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The present study aimed to develop and validate a conversion SIDA method that determines total B12 as CNCbl after cyanidation conversion and to further compare the developed method with a previously developed multi-SIDA method targeting individual native vitamers (hereafter referred as native SIDA method)

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FIGURE 2 The GF protocol produced HLCs with a mature differentiated morphology

The choice between them boils down to what the medications guidelines require and patient safety considerations: Use sterile (preservative-free) water when indicated for sensitive situations (newborns, intrathecal use, single-dose needs), and use bacteriostatic water when a multi-dose solution is needed and its safe to do so