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Samples were adjusted with 25 l 10 x lysis buffer (final concentrations 4% SDS, 100 mM HEPES, pH 8.5, 50 mM DTT) and homogenized using a Precellys 24 tissue homogenizer (Bertin Technologies) for 3 cycles (6000 mixes for 20 s followed by 30 s pause)

ROS production was higher in SRF3 -OE plants than in EV plants (Fig

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Besides, except for iron chelators DXZ and deferiprone (DFP) authorized by FDA are used in treating DIC and AMI (75, 76), a majority of researches of ferroptosis in cardiovascular diseases have only been confirmed in the cell and animal models, with relatively limited clinical evidence