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After Coomassie Brilliant Blue staining, five equal parts of the gels, divided by molecular weight, were evaporated using acetonitrile and reconstituted in 1% (v/v) formic acid, then digested with trypsin and injected into the mass spectrometer (Model LTQ, Thermo Fisher Scientific, Waltham, MA, USA)

Neuroinflammation in aging is characterized by the accumulation of many cytokines, in which the most obvious change is IL-2 production 64

however, regulatory T cells (Tregs) rely on GPX4 to resist oxidative stress after activation