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Cells were harvested by centrifugation and resuspended in lysis buffer (20 mM Tris-HCl, pH 8.0, 300 mM NaCl, 5% glycerol)

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APAP metabolism can be divided in three phases ( Figure 1 ): (1) The major bulk of APAP (8590%) is metabolized by UDP-glucuronosyl transferases (UGT) and sulfotransferases (SULT), with conversion to glucuronidated (5660%) and sulfated (2830%) non-toxic metabolites, which are excreted through the urine (McGill and Jaeschke, 2013)

10.1016/j.foodchem.2016.11.063 25 GuoZ.ChengJ.ZhengL.XuW.XieY

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