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Cells were then centrifuged at 16,000 g for 10 min at 4 C and the supernatant was transferred to liquid chromatography-mass spectrometry (LC-MS) glass vials

This reactive stroma substantially modifies the mechanical and biochemical properties of the TME, creating a favorable condition that supports tumor growth and immune evasion

In this paradigm, aquaporin 8 silencing, which is specifically expressed in IMM, enhances mitochondrial ROS generation and results in mitochondrial depolarization and cell death (Marchissio et al., 2012)

Alterations in the normal function of excitatory amino acid carrier 1 affect cysteine transport and GSH synthesis impairing zinc homeostasis (the thiol group of GSH can function as a principal Zn 2+ chelator for the maintenance of Zn 2+ homeostasis in neurons) and oxidative stress, enhancing susceptibility to ischemia-induced neuronal cell death in the hippocampus (162164)

Exposure to reactive oxygen or nitrogen species is causatively linked to the disease pathologies associated with redox imbalance

Conclusion: The addition of durvalumab to gemcitabine and cisplatin did not lead to immune mediated side effects in this real-world cohort