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and YD9100002005 to H.Z.), CAS Project for Young Scientists in Basic Research (YSBR-013 to W.X.), CAS Collaborative Innovation Program of Hefei Science Center (2021HSC-CIP003 to W.X.), University Synergy Innovation Program of Anhui Province (GXXT-2022-033 to H.Z.), Research Funds of Center for Advanced Interdisciplinary Science and Biomedicine of IHM (QYZD20230011 to W.X.) and Major Science and Technology Special Projects, Anhui Provincial Department of Science and Technology (202303a07020005 to W.X.)

223 SIRT4 modulates mitochondria energy homeostasis and longevity based on its lysine deacetylase, lysine deacylase, lipoamidase and ribosylase activity

For the GST-tag purification a second denaturing elution from the beads with 60 l SDS-PAGE sample buffer was performed after the beads had been washed with 500 l wash buffer

PMID: 19996205

Protein crystallization and structural analysis HT protein (~1 mg ml 1 ) was reacted with the respective ligand (1.5 excess) in PBS at 20 C for 1 h

[Please see additional research results in the attachment] -Use rate of 10% combined with 5% urea at 6 weeks reduces the symptoms of Type 1 Lamellar Ichthyosis