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These effects were generally mild to moderate in intensity and were most frequent during the dose escalation period, becoming less common as participants remained on their maintenance dose

After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

In the human AD brain GPX4 is downregulated at an early stage of the pathology, while upregulated at a later stage of the pathology [17]