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The effects of Hsp70 open a potential alternative treatment for vitiligo patients [84]

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Mast Cell Culture and Degranulation Assay RBL-2H3 cells purchased from the American Type Culture Collection (CRL-2256), were grown in high-glucose Dulbeccos modified Eagles medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin G, and 100 g/mL streptomycin at 37C in an atmosphere of 5% CO 2

Preparation: use appropriate sterile solvents/buffers selected by your protocol

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