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Loss of HIF1A from pancreatic cancer cells increases expression of PPP1R1B and degradation of p53 to promote invasion and metastasis

[DOI] [PubMed] [Google Scholar] 108.Schaafsma S.M., Pfaff D.W

Data were fitted by one-phase decay, dotted lines represent 95% confidence interval (CI)

10.1155/2014/238463 54 ChoiJ.KangJ.KimT.NehsC

There is also a critical need for well-designed long-term safety studies addressing the potential side effects of chronic polyphenol supplementation, as current data are mainly limited to short-term animal experiments or isolated compounds, and results from these do not always translate directly to humans (121)

Lysis solution was prepared with Nuclei PURE lysis buffer (NUC-201, Sigma-Aldrich), 1 mM dithiothreitol (D9779, Sigma-Aldrich), and 0.1 % Triton X-100 (NUC-201, Sigma-Aldrich) according to manufacturer protocol, and an RNase inhibitor mix (0.04 U/L SUPERaseIN RNase Inhibitor [AM 2696, Thermo Fisher]