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The supplementation should begin preoperatively (often 24 weeks prior) to build up nutrient reserves and continue through the postoperative period until initial healing milestones are passed (for bone, this might be 612-week post-surgery, corresponding to early consolidation of bone) [163]

Consequently, the construction procedures of the noise-induced tinnitus mouse model in our research are as follows: bilateral ears of anesthetized C57BL/6J mice were exposed to 8 kHz narrowband noise at a sound pressure level of 100 dB for 60 min, followed by a 2-hour silent period in a soundproof chamber

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