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At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min

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Due to the distinct position of the conjugated N-terminal lipid between Cagri-CTR and Cagri-AMY 1 R, we also determined a structure of CTR-Gs in complex with an analogue of Cagri that lacked the lipid modification (CagriBB) to interrogate the contribution of the acylation to the conformation of Cagri-CTR complex

In laboratory models, it is frequently investigated alongside other metabolic analytes to see how they work together to synchronize neuroendocrine signals