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RNA stability assays Actinomycin D (Act D) (Sigma Aldrich, St

After ultrasonic lysis, the supernatant samples were divided into IP, IgG, and Input, where Input was preserved at -20 and the other two were incubated overnight with the antibodies H3K27ac (5 l, CST, #8173) and TFAP2A (5 l, Affinit, AF5323)

Kidney fibrosis is hallmark of CKD, and preclinical studies have suggested that the overexpression of platelet-derived growth factor (PDGF) receptor- in mesenchymal cells is associated with glomerular and interstitial fibrosis and inflammation as well as tubular atrophy 28,29

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