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No clear evidence defines the ideal level within the normal range

A minimum of 600 cells per condition were analyzed in most experiments

KimWZhouJQHorvathSCSchmitzAJSturtzAJLeiTet al

For suspected macro-B12, precipitation with PEG is the test most often reached for, though it is nonspecific and can remove B12 that is fully available to cells, so direct measurement of the binding proteins, haptocorrin and transcobalamin, may be more informative when it is available

Low B12 can happen for different reasons

An additional feature of mRNA and viral vector vaccines is efficient induction of T-cell immunity and not only humoral responses