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Transblotting of the proteins was performed on polyvinylidene difluoride membranes (Merck Millipore) blocked in Tris-buffered saline with 5% skim milk and 0.1% Tween 20 for 1 h and incubated overnight with primary antibodies against IL-1 (1:2,000

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Figure 2 summarizes the input and output technologies utilized in OoC platforms, illustrating the multifaceted approach for the fabrication and integration of monitoring and analysis tools within these advanced in vitro models

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The difference between adding 1 mL and 2 mL of bacteriostatic water changes everything about how you measure your doses, how long your vial lasts, and whether your syringe markings actually correspond to the milligrams you think you are injecting