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Some of the labeling scenarios required to solve the HIV Nef structure are presented in Table 1 of Grzesiek et al

Animals were crashed by homogenization (1:10, w:v in 50 mM potassium phosphate buffer, pH 7.0, 0.5 mM EDTA, 1 mM PMSF) and centrifuged (8,000 g, 15 min, 4 C)

During instances of tissue damage or exposure to cytokines, cells liberate arachidonic acid from their membranes, setting off a cascade of enzymatic transformations

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The treated sugar solution was prepared by diluting ATQ in a 10% sucrose solution, resulting in a final concentration of 100 M [3]