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Regeneration of other antioxidants When an antioxidant scavenges a free radical, it becomes oxidized itself and is not able to scavenge additional ROS or RNS until it has been reduced

Samples were then diluted with SDS-free TUBE PD lysis buffer, thus decreasing the SDS concentration to 0.1%, and then incubated with glutathione sepharose beads (Cytiva) freshly pre-coupled with GST-TUBE (20 g per sample) overnight at 4 C

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The sudden influx of a high-dose substance is often unnecessary and carries more risks than a steady, controlled nutritional approach