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Notably, HIF-1 mediates adaptive metabolic responses to hypoxia by enhancing glycolytic pathway, serine synthesis and one-carbon metabolism to promote mitochondrial antioxidant production (NADPH and GSH), and inhibiting the TCA cycle so as to diminish mitochondrial ROS production (247)

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Taken together with our metabolomic profiling of L-cystine starved cells (Fig

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Thus, 1000 g/mL EDTA was used in further stability testing