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Benzodiazepines are often sought by individuals who abuse drugs and other substances, and by individuals with addictive disorders [see Warnings and Precautions (5.2)]

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doi: 10.3389/fnmol.2022.1014251 63

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For example, in the case of the E25 to M569 N-terminal deletion mutant, the following primers could be used to amplify a cDNA fragment corresponding to this deletion mutant: For example, in the case of the M1 to N500 C-terminal deletion mutant, the following primers could be used to amplify a cDNA fragment corresponding to this deletion mutant: Representative PCR amplification conditions are provided below, although the skilled artisan would appreciate that other conditions may be required for efficient amplification

This might be due to the conjugated double bonds formed by alkaline crosslinking that interfered with the electron density cloud between collagen molecules