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Samples were centrifuged at 538 g for 10 min at 4 C in a Megafufe 1.0 R centrifuge (Heraeus), and the supernatant (gut wash) was collected for further analysis

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a larger Intramuscular (in the muscle) 25 gauge 1 inch or 1.5 inch needle for maximum intramuscular benefit and a smaller Deep Subcutaneous (under skin) 28-30 gauge inch needle or 25g 5/8 inch

If you dont get enough vitamin B12 or you have low B12 absorption, your body will start to respond

Methods: We included 108 probands with a clinical phenotype of monogenic diabetes and 86 relatives for molecular analysis of 11 genes using Sanger sequencing