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Description

Induction of phase 2 antioxidant enzymes by broccoli sulforaphane: perspectives in maintaining the antioxidant activity of vitamins a, C, and e

doi:10.1093/eurheartj/eht042

Deacetylation of the peptide substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the deacetylated peptide from aminoluciferin, which is quantified in a luciferase-based reaction

The widely accepted theory is the oxidative stress hypothesis (Ghezzi et al., 2017) that advanced and modified the free radical theory of aging (Harman, 1956)