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The labeled cDNAs were used to detect marker genes of signal transduction pathways on microarray membranes using Human Signal Transduction PathwayFinder RT 2 Profiler PCR Array (SuperArray System, Bioscience, Frederick, MD, USA)

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If both beta genes are mutated with mild variants, the result is Beta Thalassemia Intermedia, characterized by moderate anemia that may require occasional transfusions and carries a risk of iron overload from increased absorption

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