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Linearity HPTLC method After establishing the stock standard solutions (1 mg mL 1 ), we injected precise dilutions of APAP, NAPQI, and NAC three times as bands employing the HPTLC equipment and the previously instilled chromatographic conditions

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The primary consideration is measurement precision smaller volumes (1mL) create higher concentrations requiring finer syringe gradations, while larger volumes improve ease of measurement