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After blocking with 5% BSA-TBST (Sigma), the membranes were incubated with the following primary antibodies from Abcam at 4C overnight: ER (1:1,000, ab3575), ER (1:1,000, ab3576), Ggt1 (1:1,000, ab175384), and Anpep (1:1,000, ab108310)

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The time course of macrophage polarization seen in MELC analyses was confirmed by FACS analysis of the paw tissue demonstrating that the macrophage populations in the observed areas are representative for the macrophages in the inflamed paw (Fig 1EH)

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However, the mechanism by which different GRK4 polymorphisms affect enzymatic activity needs more investigation in future studies