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DLS analysis indicated a PDI of 0.09 with Zeta potential value of 49.2 2.9 mV, confirming high stability

EMBO J (2000) 19:177783

doi: 10.1039/d1fo01073e 150 HanZCenCOuQPanYZhangJHuoDet al

Behavioral analysis of bitter taste perception in Drosophila larvae

Protein extraction and western blotting For protein isolation, hDFs, iPSCs and MNPs were washed twice with ice-cold 1X phosphate saline buffer (1X PBS) and lysed with an extraction buffer containing 50 mM HEPES (Thermo Fisher Scientific), 150 mM NaCl (Applichem), 1 mM EDTA (pH8) (Applichem), 1 mM EGTA(pH8), 10% glycerol (Romil Pure Chemistry), and 1% Triton-X-100 (Applichem) complemented with protease (Sigma-Aldrich) and phosphatase inhibitors (PhosSTOP, Roche), according to the manufacturers protocol

Some mushroom varieties contain more than 1,000 milligrams of glutamates per kilogram, making them among the most flavour-rich vegetables available